Bioassay-driven purification

Fig. 19.1 Flowchart of the bioassay-driven purification of chemical signals. Reproduced from Hardege (1999) with kind permission from Springer Science+Business Media...

Using a bioassay-driven purification strategy (Fig. 19.1), a number of sex pheromones were identified including such diverse molecules as ketones, uric acid, peptides, and L-ovothiol-A (Zeeck et al. 1988 Rohl et al. 1999 Ram et al. 1999). This has led to recent studies investigating the biological functions of pheromones, such as mate tracking and mate choice (Ram et al. 2008). 

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