Bioanalytical Mass Spectroscopy

Computational methods including both metabolism databases and predictive metabolism software can be used to aid bioanalytical groups in suggesting all possible potential metabolite masses before identification by mass spectroscopy (MS) [116,117]. This approach can also combine specialized MS spectra feature prediction software that will use the outputs from databases and prediction software and make comparisons with the molecular masses observed... 

Anker J, Hall W, Lambert M, Velasco P, Mrksich M, Klein W, Van Duyne R (2009) Detection and identification of bioanalytes with high resolution LSPR spectroscopy and MALDI mass spectrometry. J Phys Chem 113 5891-5894. doi 10.1021/jp900266k... 

High-performance liquid chromatography (HPLC) has emerged as one of the most useful tools for the bioanalytical laboratory. This is a powerful stand-alone method that can be used for the purification, separation and quantification of biomolecules. Notably HPLC analysis can be combined with other high-tech methods such as mass spectrometry (MS) or nuclear magnetic resonance (NMR) spectroscopy to enable the identification of biomolecules. 

Bioanalytical Separations, ed. I. Wilson, in Handbook of Analytical Separations, Vol. 4, Elsevier Science B.V., 2003 R 138 J.C. Lindon, NJ.C. Bailey, J.K. Nicholson and I.D. Wilson, Biomedical Applications of Directly - Coupled Chromatography - Nuclear Magnetic Resonance (NMR) Spectroscopy and Mass Spectrometry (MS) , p. 293... 

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