Binding Helmholtz Energies on First and Second Sites

Binding Helmholtz Energies on First and Second Sites 

As in subsection 3.2.4, we now define the binding Helmholtz energies on first and second sites. First we bring a ligand G from a fixed position in vacuum onto a specific site, say on the Ihs site of an empty polymer. The process is symbolically written as 

Note that in the definition of we used the ratio of the canonical PFs Q 0) and 

However, if we take the open system PF (any A), then from (3.3.27) we have 

Next we define the binding process to the second site, which is symbolically written 

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Binding Helmholtz Energies on First and Second Site, Cooperativity... 

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