Probe sequences, binary probes

The noncovalent specific labeling of NA probes based on the aptamer strategy has also been reported. The malachite green (37) aptamer, which was designed as a binary probe, assembles only in the presence of the target sequences and induces an increase in the intensity of the signal emitted by the F bound aptamer. 

MGA has a stem-loop structure containing internal bulged loops [75]. These double-stranded sequences were separated into two single-strand sequences, each of which had no affinity to malachite green, and were tagged to sequences complementary to nucleic acid analytes. This probe is referred to as a binary MGA probe, and provides immediate fluorescent response after hybridization to complementary nucleic acid analytes, thus offering easy and instant detection of specific DNA and RNA [78]. 

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