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Bimolecular fluorescence complementation BiFC-based biosensors

Type 1 Forster (or fluorescent) resonance energy transfer (FRET)-based biosensors Type 2 Bimolecular fluorescence complementation (BiFC)-based biosensors Type 3 Single FP-based biosensors... [Pg.29]

Bimolecular Fluorescence Complementation (BiFC)-based biosensors... [Pg.30]

Bimolecular fluorescence complementation (BiFC)-based biosensors have been used to visualize a variety of protein—protein interactions in live cells. In this type of biosensor, the FP that is split up and MRE are linked to one portion and the analyte protein is linked to the other portion. When the two proteins interact, the two fragments fuse together, refolding properly into its three-dimensional structure and producing a fluorescence signal. [Pg.30]

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