Bile, microdialysis

Microdialysis sampling has been used in rats infused with phenol (0.181 nmol/minute for 90 minutes) to study excretion into the bile (Scott and Lunte 1993). For all phenol metabolites, bile concentrations were higher than liver concentrations indicating that the metabolites are actively excreted in the bile. 

Scott DO, Lunte CE. 1993. In vivo microdialysis sampling in the bile, blood, and liver of rats to study the disposition of phenol. Pharmaceutical Res 10 335-342. 

Figure 2 Typical microdialysis probe geometries, (a) Rigid cannula probe typically used for implantation in the brain, (b) Flexible cannula probe for implantation in the blood vessel of a rat. (c) Linear probe designed for peripheral tissues such as skin, muscle, or liver, (d) Shunt or bypass probe is a linear probe inside a larger tube used for sampling the bile duct of the rat.

Figure 12 Typical microdialysis probes for various tissues of rats or similar-size animals. (A) intracerebral guide cannula and rigid concentric cannula probe for brain tissue, (B) linear probe used for sampling peripheral tissues such as liver, muscle, dermis, tumor, kidney, (C) vascular probe for sampling from the jugular vein, and (D) shunt probe for sampling from the bile duct. Unlabeled arrows indicate direction of dialysate flow into and out of probes. (Courtesy of Bioanalytical Systems Inc.)...

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