Bile acid conjugates, quantitation formation

If urine is acidified (pH 1) and then extracted three times with equal volumes of /i-butanol, bile acid conjugates can be quantitatively recovered (28). However, due to the time-consuming evaporation of n-butanol and the formation of emulsions other ways to extract bile acids have been tried. The method described by Hofmann (21) may be useful. 

---