Biased signaling

PCAs facilitate analysis of formerly unassayable processes like ubiquitinylation and histone (de-)acetylation in a live-cell context (4). It also significantly broadens the spectrum of assays around well-known targets such as GPCRs enabling analysis of GPCR homo-and heterodimerization and biased signaling (5). 

Figure 1 Bias in the chemokine system and other 7TMRs. Biased signaling describes the ability of a receptor to induce different signaling pathways or cellular events. Thereby, different ligands may activate different pathways via the same receptor (ligand bias, A), or the same ligand induces different outcomes at different receptors (receptor bias, B). Also the cell or tissue that "hosts" the ligand receptor interaction can modulate the induced signaling pathway (tissue bias, C).

Corbisier, J., Gales, C., Huszagh, A., Parmentier, M., Springael, J. Y. (2015). Biased signaling at chemokine receptors. The Journal of Biological Chemistry, 290, 9542—9554. 

Namkung, Y., Radresa, O., Armando, S., Devost, D., Beautrait, A., Le GouiU, C., et al. (2015). Quantifying biased signaling in GPCRs using BRET-based biosensors. Methods. http //dx.doi.Org/10.1016/j.ymeth.2015.04.010. [Epub ahead of print]. 

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