Berry spot test

The Berry spot test provides a rapid qualitative evaluation of urine. GAGs react with toluidine blue, a cationic dye, to yield a pink-colored compound. Alternative spot tests have been published but suffer from the same drawbacks regarding false-negative and false-positive specimens [2, 10, 15, 34]. Nevertheless, this fast procedure may pick up patients who have been referred to a laboratory for other metabolic examinations. For specific, initial MPS testing, the 1,9-dimethylene blue (DMB) assay, described below, is recommended. [Pg.291]

Fig. 4.1.5 Examples of a positive (a) and a negative (b) Berry spot test from urine. The filter paper will remain light blue after washing. The pink spot is typical for elevated glycosaminoglycan (GAG) excretion...

The specimen requirements are the same as for the Berry spot test. [Pg.294]

Berry HK (1987) Screening for mucopolysaccharide disorders with the Berry spot test. Clin Biochem 20 365-371... [Pg.321]

Mabe P, Valiente A, Soto V, Cornejo V, Raimann E (2004) Evaluation of reliability for urine mucopolysaccharidosis screening by dimethylene blue and Berry spot tests. Clin Chim Acta 345 135-140... [Pg.323]

Berry HK, Berry MA, Spinanger J (1960) A paper spot test useful in study of Hurler s syndrome. J Lab Clin Med 55 136-138... [Pg.321]

Chih-Kuang C, Shuan-Pei L, Shyue-Jye L, Tuen-Jen W (2002) MPS screening methods, the Berry Spot and acid turbidity tests, cause a high incidence of false-negative results in San-filippo and Morquio syndromes. J Clin Lab Anal 16 253-258... [Pg.322]

Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...