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Bead probes fluorescence emission

Fluorescence Emission Light Scatter Nanoparticle-Bead Probes... [Pg.17]

PSF can be measured experimentally in a simple manner involving the acquisition of images of subresolution fluorescent beads. These microbeads, commercially available as a concentrated solution, are used to represent a point source for evaluation of the system. The smallest beads possible should be used in theory, but small beads are weaker in intensity and bleach more rapidly. In practice, beads with a diameter less than half the resolution are used beads of 0.15 pm in diameter, from Molecular Probes Inc., for a 1.4 NA oil immersion objective with a lateral resolution of 0.4 pm at an emission wavelength of 500 nm. Beads are diluted to isolate each PSF without overlap, and are dried onto the coverslip. The coverslip is then reversed, mounted in the same medium as used... [Pg.211]

See other pages where Bead probes fluorescence emission is mentioned: [Pg.494]    [Pg.1]    [Pg.95]    [Pg.266]    [Pg.102]    [Pg.165]    [Pg.391]    [Pg.416]    [Pg.213]   
See also in sourсe #XX -- [ Pg.17 , Pg.18 , Pg.19 , Pg.20 , Pg.21 , Pg.22 , Pg.23 , Pg.24 , Pg.25 , Pg.26 ]



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