Basic Analytes loading capacity

Increasing the load of basic analytes in order to increase analyte sensitivity can lead to a decrease in apparent peak efficiency and increase in peak tailing. However, if an analysis must be performed at a relatively high sample load, the addition of a chaotropic additive may be employed to increase the apparent peak efficiency and symmetry. Much higher loading capacities could be obtained by operating columns with these mobile-phase additives without substantial deterioration in efficiency. 

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