Barley lectin protein

Fig. 4. Immunoprecipitation of AB A-induced proteins in barley aleurone layers with antibodies against barley lectin and a-amylase inhibitor. Aleurone layers were incubated with or without 2 X 10 M ABA for 24 h. Labeling with 50 jaCi/ml p S] methionine took place in the final h of incubation. In A proteins were extracted with 0.1 M Na acetate (pH 5.5) in B proteins were extracted with 2% Triton X-100 at 40 C for 30 min. Aliquots of the extracted proteins were reacted with the antibodies and then precipitated with Staphylococcus aureus Cowan strain 1. The pellets were washed and analyzed by SDS-PAGE. Fluo-rograms of the gels are shown. Mol wt marker are indicated by bars at left. L.-S. Lin and T.H.D. Ho, unpublished...

---