Bacteriophage bound

Figure 16.19 Schematic drawing illustrating the structure and sequence of the RNA fragment that is recognized and bound by the coat protein of bacteriophage MS2. The RNA fragment forms a base-paired stem with a bulge at base -10 and a loop of four bases. Bases that form sequence-specific Interactions with the coat protein are red. (Adapted from a diagram provided by L. Llljas.)...

Fig. 7.11. Representation of the high-resolution structure of the bacteriophage MS2 coat protein (magenta) bound to an eighteen nucleotide RNA aptamer (cyan) at 2.8A resolution [112]. The MS2 coat protein is fused to the DBD in the RNAY3H system and is used to anchor the RNA sequence at the promoter region [90],...

Figure 27.38. Holliday Junction. (A) The structure of a Holliday junction bound by Cre recombinase (gray), a bacteriophage protein. (B) A schematic view of a Holliday junction.

Figure 3-16 Model of bacteriophage fd engineered to display peptides as inserts in the coat proteins of the virus. The native virus structure is shown in gray proteins not present in the native virus are shown black or green, inserted near the N-termini of some major coat proteins is a 6-residue peptide. To one of these peptides a specific Fab antibody fragment (green) has bound from solution, and a second Fab is shown nearby. The N-terminal region of a minor coat protein at the end of the virion has been engineered to display a (different) Fab fragment. Steric constraints are less stringent for inserts in the minor proteins, but fewer copies per virion are possible. Reprinted with permission from Barbas, et...

Fig. 8.5 High-throughput screening at 10mMforWBZ 4 (red) andimatinib (STI 571, blue, control) over a battery of human kinases displayed in a T7-bacteriophage library (Ambit Biosciences, San Diego, CA). Hit values are reported as percentage-bound kinase. Reprinted from the [14], copyright 2007 with permission from the American Society for Clinical Investigation...

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