Bacterial alkaline phosphatase requirements

Metal ions also participate in the functioning of other nucleases, although the structural details of their participation are not nearly as established as those for staphylococcal nuclease. DNAse I also requires Ca for its catalytic activity.SI endonuclease, mung bean nuclease, and Physarum polycephalem nuclease require zinc ion either as cofactors or intrinsically for nuclease activity, and the restriction enzyme EcoRI may also require intrinsically bound zinc ion. In terms of how the zinc ion might function in these enzymes, one can look both to staphylococcal nuclease and to bacterial alkaline phosphatase for some... 

The targeting of antibodies to the periplasm requires the use of signal peptides. The pelB leader of the pectate lyase gene of Erwinia carotovora (56) is commonly used. The gill leader (9), the phoA leader of the E. coli alkaline phosphatase, and the ompA leader of E. coli outer membrane protein OmpA have also been used, being common to many protein expression vectors (57,58). Further examples are the heat-stable enterotoxin II (stll) signal sequence (47) and the bacterial chloramphenicol acetyltransferase (cat) leader (59). 

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