Bacillus putrificus

Mamoli and his colleagues also recognized a class of bacterial reductions, which they attributed to an anaerobic bacterial species identified as Bacillus putrificus. Al-... 

Sites of Reaction. Reduction of conjugated double bonds was first observed by Mamoli and Schramm (M-548) with a crude culture of putrefactive bacteria. The pure culture, described as Bacillus putrificus, reduced 3-keto-A -androstenes to 3-keto-5 -androstanes and Sa-hydroxy-S S-androstanes (M-545). This culture is no longer available (E-202), but a modern equivalent is Clostridium paraputrificum, described by Schubert (Ap-71, S-823). The properties of the latter are described in greater detail in the section on reduction of carbonyl groups. 

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