Bacillus kaustophilus

In Section 5.03.6.2, a stereoselective synthesis of L-homophenylalanine from the racemic AAacetylated amino acid is described. The authors, however, found that substrate solubility limited the utility of this procedure. Having found an L-N-carbamoylase in Bacillus kaustophilus, they introduced the gene for this enzyme together with that for the N-acyl amino acid racemase from D. radiodurans into E. coli for coexpression. These cells, permeabilized with 0.5% toluene, were able to deliver L-homophenylalanine in 99% yield and were able to be used for multiple reaction cycles. 

Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus,... 

An enantioselective synthesis of (5)-2-amino-4-phenylbutanoic acid by the hydantoinase method was developed by Hsu s group in 2003. This methodology was based on the use of a combination of Bacillus caldoly-ticus hydantoinase and Bacillus kaustophilus L-A-carbamoylase, using... 

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