Awdeh, Williamson and Askonas

If it is suspected that the results are being influenced by the polyacrylamide gel itself, then the sample can be applied at opposite ends of the gel (but not in line with each other). After running one sample, the plate should be turned end to end, and the other sample should be run in the opposite direction to the first one. If the zones obtained in the two runs do not agree as to number and relative position, it is possible that the structure of the gel is influencing the results. However, according to the authors, the gel structure most probably has no effect on the results of electrofocusing proteins with molecules of normal size. 

The layer of polyacrylamide showed a wavy structure after the run. This is probably due to variations in conductivity at different parts of 

The gels can be dried after dyeing. They can then constitute permanent records. 

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Figure 22. Apparatus for isoelectric focusing in thin layer polyacrylamide gels according to Awdeh, Williamson, and Askonas (7). (o) polyacrylamide gel 1 mm thick (b) glass plate (c) carbon electrodes 20 cm apart (d) site of sample application. Each sample, 100/400 Mg of protein in less than 50 m1, was pipetted onto the surface of the gel and spread over a rectangular area about 1X2 cm. (Awdeh el al, 39.)...

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