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Avidin-biotin horseradish peroxidase immobilization

The immobilization proceeded as follows horseradish peroxidase (HRP) was biotinilated with biotinamidocaproate AT-hydroxysuccinimide ester to obtain biotinylated HRP with two biotin molecules per enzyme molecule. Avidin was immobilized on polystyrene support beads using the carbodiimide method. This procedure was followed by an attachment of the disubstituted biotinylated HRP... [Pg.181]

Chalkias NG, Giannelis EP (2007) An avidin-biotin immobilization approach for horseradish peroxidase and glucose oxidase on layered silicates with high catalytic activity retention and improved thermal behavior. Ind Biotechnol 3 82-88... [Pg.239]

Kemp and co-workers (K6) developed a colorimetric detection system that incorporates biotin into one (nested) primer and the sequence for a DNA-binding protein (e.g., the GCN4 gene from Saccharomyces cerevisiae) into the other primer. Amplified DNA is captured on an immobilized affinity reagent and the biotinylated product is detected with avidin-horseradish peroxidase and a chro-mogenic substrate. [Pg.172]

Biotin-avidin D and biotin-streptavidin immobilization inside PDMS Glucose oxidase and horseradish peroxidase (i) 6-D-gluconolactone and hydrogen peroxide (ii) resorufin [173]... [Pg.363]

See other pages where Avidin-biotin horseradish peroxidase immobilization is mentioned: [Pg.315]    [Pg.181]    [Pg.182]    [Pg.222]    [Pg.561]    [Pg.152]    [Pg.61]    [Pg.362]    [Pg.387]    [Pg.499]   
See also in sourсe #XX -- [ Pg.332 ]



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Avidin

Avidin-biotin

Horseradish

Horseradish immobilization

Horseradish peroxidase immobilized

Peroxidases Horseradish peroxidase)

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