Autoradiography double label

Beckstead RM (1985) Complementary mosaic distributions of thalamic and nigral axons in the caudate nucleus of the cat double anterograde labeling combining autoradiography and wheat germ-HRP histochemistry. Brain Res., 335, 153-159. 

Maxam and Gilbert Method. In 1977, Allan Maxam and Walter Gilbert developed a sequencing method that replaced the plus and minus method. This method was similar, as it required a radioactive label, gel electrophoresis for fragment separation, and the use of X-ray autoradiography for product visualization and inference of the sequence. However, the method differed in that it allowed for the direct analysis of purified double-stranded DNA and used another way of creating products ending in a specific nucleotide. 

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