AutoFit peaks

AutoFit Peaks I, Residual method A residual is the difference in y value between a data point and the sum of component peaks evaluated at the data point s x value. Hidden peaks are revealed by positive residuals. 

AutoFit Peaks II, Second derivative method A smooth second derivative of the data will contain local minima at peak positions. The second derivative method requires a constant x-spacing operated in the time domain. 

AutoFit Peaks III, Deconvolution method Deconvolution is a mathematical procedure that is used to remove the smearing or broadening of peaks arising because of the imperfection in an instrument s measuring system. Hidden peaks that display no maxima may do so once the data have been decon-voluted and filtered. This method requires a uniform x-spacing operated in the frequency domain. 

Figure 5.3. Peak analysis of liquid chromatogram. The chromatographic separation of a protein sample is analyzed with PeakFit using Haarhoff-Van der Unde (HVL) function by AutoFit Peaks I menu.

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