Attachment of antigens or antibodies to plastic using bridging molecules

Attachment of antigens or antibodies to plastic using bridging molecules 

Antigens which bind poorly to plastic may be attached to the solid phase by a bridging molecule, e.g., a protein with a high affinity for the plastic to which poor binders may be conjugated by any of the methods discussed in Chapter 11. Positively charged compounds, such as protamine sulfate have been used to bind native DNA to polystyrene (see below). 

Skurrie and Gilbert (1983) used BSA as the bridging molecule for the binding of rubella antigens. BSA (fraction V) in distilled water is added to the wells and the plates are dried in a water bath at 56°C under a stream of air. An equal volume of ethanol- 

Covalent linking of antibodies to polystyrene plates (Neurath and Strick, 1981) 

Fill in a well-ventilated hood the wells of the microplate with methanesulfonic acid and incubate overnight at room temperature. 

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