Assays Based on Frustrated Super-Quenching

The principle of frustrated super-quenching as a detection technique for quantifying the catalytic activity of hydrolytic enz3mies can also be extended by using solid supported polymer and enveloping it with anionic biopolymers like carboxymethyl amylose (CMA) or carboxymethyl cellulose (CMC) to shield its fluorescence from electron transfer or energy transfer quenchers. 

As described in Sect. 2.1, cyanine dyes can form J-aggregates that are characterized by sharp red-shifted absorbance and fluorescence compared with the monomer. As discussed in the introduction, J-aggregates can form on inorganic nanoparticles or microspheres as well as within polymeric ensembles. Other studies have indicated that cyanines can self-assemble on biopolymeric scaffolds to form aggregates with properties that depend on the structure of the host. 

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