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Assay strategies antibodies

This chapter gives an overview of the current knowledge about the immunogenicity of therapeutic proteins. First the mechanisms by which antibodies are formed will be discussed, followed by the factors that can influence the immune response. An assay strategy for the detection of antibodies in human sera is discussed. The... [Pg.816]

In these experiments, a uniform ratio and concentration of DNA and STV was established for the 227-bp DNA marker, indicating a successful strategy for the development of various IPCR applications by standardized assay conditions for these compounds. In contrast, the concentration of the antigen-specific biotinylated antibody was adapted for each assay. [Pg.282]

Recently, two different strategies were developed for a competitive IPCR of small molecules, such as, for example, hormones, messengers, or transmitters without multiple antibody-binding sites necessary for sandwich assays. [Pg.283]


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Assay strategies

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