Arginine-Reactive and Photoreactive Crosslinkers

ASBA has been used to identify parasite adhesive proteins (Gowda et al., 2007), for active site-directed labeling of glucosidase I (Romaniouk et al., 2004), and to study interactions with the proteasome (Qureshi et al., 2003). 

The ability to direct conjugation or modification specifically through arginine residues using this chemistry has been exploited in the availability of the only photoreactive glyoxal derivative, APG. 

p-azidophcnyl glyoxal, is a heterobifunctional crosslinker containing an arginine-specific diketone group on one end and a photosensitive phenyl azide group on the other end (Thermo 

A convenient molecule from which to build trifunctionals is the amino acid, L-lysine. Its three functional groups, a-carboxy, a-amino, and e-amino, can be derivatized independently to contain three arms. Each arm can be designed to terminate in a complexing group able to participate in a particular type of conjugation reaction or affinity interaction. 

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