Aqueous solution infrared spectroscopy peptides

Analysis of the spectrum of CiT4 in TFE/D2O solution indicates differences in the secondary structure of the peptide when compared to that observed for the peptide in the pure aqueous solution. On addition of TFE, a more structured conformation of CiT4 is indicated. Notably, a-he ical structures and -structures appear to be formed in the less polar environment provided by the TFE. Thus, a change in environment, in this case the solvent, can cause notable changes to the peptide conformations which are observable when using infrared spectroscopy. 

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