Aptasensors principles

Figure 12.5 Principles of the two types of impedimetric aptasensors in TREAS mode. (A) An aptamer-containing strand is immobilized onto the electrode (B) A partlially complementary strand (PCS) is immobilized onto the electrode ABA represents the adenosine-binding aptamer. [(A) From (Zayats et al. 2006) (B) from (Li et al. 2007b).]...

Figure 12.7 Principle of the multifunctional aptasensor. ABA, ATP-binding aptamer TBA, a-thrombin-binding aptamer MBA, strand containing the aptamers PCS, strand partly complementary to MBA. [From Du et al. (2008).]...

Fig. 14 Schematic diagram for the principle of the developed ECL aptasensor for detecting thrombin. (A) The adsorption of thiolated antithrombin aptamer on and the 2-mercapto-ethanol block to the electrode. (B) The formation of the dsDNA between aptamer and its complementary ssDNA. (C) The intercalation of Ru(phen)3 into the dsDNA sequence. (D) Dissociation of dsDNA and release of Ru(phen)3 due to the interaction between thrombin and its aptamer. Reprinted with permission from Ref 97. Copyright (2009) American Chemical Society.

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