Aptamer-Modified Surfaces for Affinity MALDI-MS

In the following sections we describe our results for affinity MALDl-MS of two proteins, thrombin and immunoglobulin E (IgE). IgE provides a good contrast to thrombin in terms of both size (thrombin is 36kDa and IgE is 

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Our first demonstration of aptamer-modified surfaces for affinity MALDI-MS used the thrombin-TBA system described for affinity CE studies in the preceding section (Dick and McGown, 2004). In the MALDI-MS experiments, 3 fiL of sample was applied to the aptamer-coated and scrambled oligonucleotide-coated MALDI probe surfaces and incubated for 30 minutes. The surfaces were then rinsed with buffer, followed by application of the MALDI matrix and MALDI-TOF-MS analysis. 

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