Application of Quenching to Protein Anisotropy Decays

While the use of glc al analysis with quoiching is us ul for studies of rigid anisotropic rotors Ais method is even more useful in studies of complex anisotropy decays from proteins. This is because the correlation times for segmra-tal motions and overall rotations of a protein are usually quite different in magnitude, whereas the correlation dmes of a rigid rotor are usually similar in magnitude. 

The existence of two rotational motions is easily visible in the frequency response of the polarized emission. These 

Because of the conoplexity of the theory, one often encounters various sinyitified forms. When the absocpdon and emission tranntion momenb arecollinear. and perpendicular to the hdix axis, the aniscrtropy decay is given by  

Many fluorophores intercalate into double-helical DNA. Some fluorophores. like BB. become more highly fluorescent when bound to DNA. allowing the nission of the DNA-bound probes to be easily detected. Consequently, there have been numerous studies of the anbotropy decays of DNA-bound probes. At first glance, a DNA-bound probe seems rdatively single. Suf iose that the probe is 

Hpire 12 2. Flmteaoeace uisotro decqr curves of tatfwaUsted EB in clwonialin IWA. Buflinr ooodaioos wen tmM Itis Md 02aM 

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