Application of DIOS in Bioanalysis

A further advantage, as described by Thomas et al. [19], is the possibility of protein identification that follows the functional characterization of the enzyme. The activity of an enzyme is initially determined by following the substrate consumption and product formation in the first assay (Fig. 8.10). Since no matrix components are present in the sample spot, the immobilized enzyme is then directly 

The surface of the porous silicon chip offers multiple possibilities for the covalent coupling of functional groups. Thus, functionalization of a DIOS target by immobilization of trypsin was described by Xu et al. [21]. The enzyme was immobilized using cyanuric chloride as coupling agent following an amino-functionalization (Fig. 8.11). 

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