Application of C3 Exoenzyme in Biological Systems

The biological effects of C3 exoenzyme correlate well with the extent of in sifu ADP-ribosylation of Rho. However, the biological effect does not vary linearly with the extent of ADP-ribosylation, but is 

Preparation of Clostridium botulinum C3 Exoenzyme and Application of ADP-ribosylation of Rho Proteins in Biological Systems 89 

Although C3 exoenzyme affects many biological responses caused by extracellular stimuli, presumably due to the ADP-ribosylation of Rho (Kumagai et al., 1993 Paterson et al., 1990 Ridley and Hall, 1992 Mabuchi et al., 1993 Kishi et al., 1993 Tominaga et al., 1993 Takaishi et al., 1993 Hirata et al., 1992 Yamamoto et al., 1993 Hill eta/., 1995 Kumagai etal., 1995), there has been no proper con- 

Aktories K, Weller U, Chhatwal GS (1987) Clostridium botulinum type C produces a novel ADP-ribosyltransferase distinct from botulinum C2 toxin. FEES Lett. 212  

Chardin P, Madaule P, Tavitian A (1988) Coding sequence of human rho cDNAs clone 6 and clone 9. Nucleic Acids Res. 16 2717-2722. 

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