Apoglucose oxidase

A homogeneous electrochemical enzyme immunoassay for 2,4-dinitrophenol-aminocaproic acid (DNP-ACA), has been developed based on antibody inhibition of enzyme conversion from the apo- to the holo- form Apoglucose oxidase was used as the enzyme label. This enzyme is inactive until binding of flavin adenine dinucleotide (FAD) to form the holoenzyme which is active. Hydrogen peroxide is the enzymatic product which is detected electrochemically. Because antibody bound apoenzyme cannot bind FAD, the production of HjOj is a measure of the concentration of free DNP-ACA in the sample. 

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A homogeneous enzyme immunoassay based on antibody inhibition of enzyme conversion from the apo- to the holo- form has been developed for DNP-aminocaproic acid, DNP-ACA (Ngo et al., 1985). A competitive equilibrium is established between the analyte DNP-ACA and DNP-conjugate apoglucose oxidase, DNP-CAGO, which is the labeled hapten. Flavin adenine dinucleotide, FAD, added to the mixture binds to free DNP-CAGO to give DNP-CAGO FAD, which is enzymatically active and... 

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