APC Genotyping in Min Mice

Min mice have a truncating mutation in the murine APC gene at a position similar to that found in many FAP patients and develop multiple intestinal adenomas [24]. Heterozygous APC mice were therefore used to evaluate the efficacy of potential chemopreventative compounds on development of intestinal polyps [25]. The compounds tested were sulindac, a non-steroidal anti-inflammatory drag with estabUshed chemopreventative activity, and EKB-569, an irreversible inhibitor of the epidermal growth factor receptor kinase. 

Alleie Sequence Molecular Mass M/z of ion monitored Daughter ions monitored 

Polyp formation was significantly reduced with sulindac alone ( 70% reduction with a dose of 20mg/kg) and EBCB-569 (87% reduction with a dose of 20mg/kg). Moreover, when a lower dose of sulindac was administered in combination with EKB-569, 95% reduction in polyp number was observed [25]. These results 

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