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Polyethylene glycol antibody immobilization with

Fig. 14.4. Indirect EIA with or without polyethylene glycol (PEG) 6000. Virus was immobilized and antiserum was added with or without PEG 6000 (left frame) and detected subsequently with peroxidase-conjugated anti-IgO antibodies (right frame) with or without PEG 6000. The threshold activity obtained after various incubation periods of the solutions was determined. It can be seen that PEG 6000 has no beneficial effect on the first reaction, in contrast to the subsequent reaction(s). Fig. 14.4. Indirect EIA with or without polyethylene glycol (PEG) 6000. Virus was immobilized and antiserum was added with or without PEG 6000 (left frame) and detected subsequently with peroxidase-conjugated anti-IgO antibodies (right frame) with or without PEG 6000. The threshold activity obtained after various incubation periods of the solutions was determined. It can be seen that PEG 6000 has no beneficial effect on the first reaction, in contrast to the subsequent reaction(s).

See other pages where Polyethylene glycol antibody immobilization with is mentioned: [Pg.5996]    [Pg.16]    [Pg.652]    [Pg.15]    [Pg.45]    [Pg.188]    [Pg.47]    [Pg.192]    [Pg.706]    [Pg.337]   
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