Antibodies reductive amination with

Figure 7.11 Oxidation of glycoproteins with periodate, such as glycosylated antibodies, results in the formation of aldehyde groups that can be used for conjugation to dendrimers containing amine groups. Reductive amination with sodium cyanoborohydride results in coupling via secondary (or tertiary) amine bonds.

Gray, G.R. (1978) Antibodies to carbohydrates Preparation of antigens by coupling carbohydrates to proteins by reductive amination with cyanoborohydride. In Methods in Enzymology, (V. Ginsburg, ed.), Vol. 50, pp. 155-160. Academic Press, New York. 

Vol. L [12]. Antibodies to Carbohydrates Preparation of Antigens by Coupling Carbohydrates to Proteins by Reductive Amination with Cyanoborohydride. G. R. Gray. 

Figure 20.14 Periodate oxidation of HRP creates aldehyde groups on the carbohydrate chains of the enzyme. Reaction with a Fab fragment then may be done using reductive amination to produce a lower-molecular-weight complex than would be obtained using intact IgG antibodies.

A second method of immunotoxin preparation by reductive amination involves the use a polysaccharide spacer. Soluble dextran may be oxidized with periodate to form a multifunctional crosslinking polymer. Reaction with antibodies and cytotoxic molecules in the presence of a reducing agent forms multivalent immunotoxin conjugates. The following sections discuss these options. 

Figure 21.15 A periodate-oxidized dextran polymer may be reacted with both an antibody and an intact toxin component using reductive amination to form a multivalent immunotoxin complex.

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