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Antibodies preserving antigen binding

Figure 20.9 Polysaccharide groups on antibody molecules may be oxidized with periodate to create aldehydes. Modification with biotin-hydrazide results in hydrazone linkages. The sites of modification using this technique often are away from the antibody-antigen binding regions, thus preserving antibody activity. Figure 20.9 Polysaccharide groups on antibody molecules may be oxidized with periodate to create aldehydes. Modification with biotin-hydrazide results in hydrazone linkages. The sites of modification using this technique often are away from the antibody-antigen binding regions, thus preserving antibody activity.
High concentrations of sodium chloride and azides are used frequently as preservatives in commercial preparations, but these components can reduce antibody reactivity. Excessive ionic strength can decrease specific staining by interfering with antibody-antigen binding. [Pg.112]

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