Antibiotics 445 Cross linked polymers

Fig. 6. A Chromatogram of a mixture containing the print molecule (oxacillin), two other p-lactam-antibiotics (penicillin G and penicillin V) and a non- 3-lactam-antibiotic (bacitracin) on an oxacillin imprinted MIP containing 4-vinylpyridine residues, cross-linked with TRIM. The analysis was performed in organic mobile phase (ACN/AcOH,99 l).B Same conditions but using the respective non-imprinted control polymer. C Structures of penicillin V, penicillin G, and oxacillin. Reprinted with permission from Skudar K, Briiggemann O, Wittelsberger A, Ramstrom O (1999) Anal Commun 36 327. Copyright 1999 The Royal Society of Chemistry...

FIGURE 44-2 Action of fi-iactam antibiotics in SUqyhytococcus aureus. The bacterial cell wall consists of glycopeptide polymers linked via bridges between amino acid side chains. In S. aureus, the bridge is (Gly),-D-Ala between lysines. The cross-linking is catalyzed by a transpeptidase, the enzyme that penicillins and cephalosporins inhibit. 

Most applications of SSI techniques have been patented [54-57] and have only been published recently in the open literature [58, 59]. The polymers used are cross-linked polyvinylpirrolidone, bovine serum albumin, polyglycolic acid, polydioxanone the solutes used for the impregnation are model molecules, antiinflammatory drugs and antibiotics (gemtamicin sulfate on bovine serum albumin). 

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