Anti-antigen antibody

The commercially available Enzygnost HBe monoclonal kit (distributed by Dade Behring) can be considered as an example for an immunoassay which combines many favorable features, e.g. nonisotopic label, sandwich immunoassay principle, use of monoclonal antibodies, determination of antigen and anti-antigen antibodies in a single test, microtiter plate format, possibility for semi- and fully automation. [Pg.654]

Mix enzyme-labeled antigen. Limited concentration of anti-antigen antibody, and sample containing free antigen... [Pg.1567]

Wash to remove unbound antigen Add enzyme conjugated anti-antigen antibody Enz... [Pg.1567]

Incubate with diluted human serum. Anti-antigen antibodies in serum are captured by the immobilized antigen... [Pg.1570]

Enzyme conjugated antibody binds anti-antigen antibody captured by immobilized antigen... [Pg.1570]

IP without the anti-antigen antibody (e.g., only with protein A sepharose or Pansorbin). [Pg.146]

Washing off of unbound anti-antigen antibodies (3 X 10 minutes). [Pg.150]

Adsorption of affinity-purified or monoclonal anti-antigen antibody to solid material (e.g., PVC microtiter plates) takes hours. [Pg.150]

Addition of an enzyme-conjugated anti-antigen antibody. With monoclonal antibodies, the first and second antibodies must not be identical. [Pg.150]

Antigen concentration —Enzyme-conjugated anti-antigen antibody 2 (constant) —Antigen (vanable) —Anti-antigen antibody 1 (constant) The assay requires two monoclonal or one affinity-purified antibody. One of the antibodies must be conjugated with enzyme. [Pg.151]

Anti-antigen antibody (screen tor hybridoma supernatants determine serum titer) —Enzyme-conjugated anti-IgG antibody (constant) —Anti-antigen antibody (variable) —Antigen (constant) Enzyme-conjugated anti-IgG antibody is available in retail. ... [Pg.151]

Antigen concentration —Enzyme-conjugated anti-antigen antibody (constant) -i- antigen (variable) —antigen (constant) Little work specific. The anti-antigen antibody must be enzyme-conjugated. Polyclonal antibodies must be affinity-purified first. [Pg.151]

Anti-antigen antibodies 1 and 2 monoclonal antibodies against different epitopes of the antigen, or one of the anti-antigen antibodies is an affinity-purified polyclonal antibody. [Pg.151]

Dot blots adsorb the antigen to nitrocellulose membranes. The dot blot distinguishes itself by quick antigen loading, and you can use serum as an anti-antigen antibody solution. The dot blot is also compatible with the 96-well microtiter plate technique. In comparison to ELISA, it requires two additional devices and steps. [Pg.151]

With dot blots and ELISAs, you can easily fall victim to artifacts and false positive signals, especially if you use serum dilutions as anti-antigen antibody solution. Controls ... [Pg.152]

Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...