Anime systems, design procedures

Parts 58.41 through 58.51 cover the physical facihties of the laboratory. The inspector must determine whether or not the facilities are of adequate size and design for completed or in-process studies. The physical parameters and systems of the facilities as they are used to accommodate the various operations employed in the GLP studies are examined. Investigators also deal explicitly with the environmental control and monitoring procedures for critical areas, especially the rooms used for animal housing, the test article storage areas, and the laboratory areas in which biohazardous material is handled. The procedures and methods for cleaning equipment and areas critical to study conduct as well as the cur-... 

Fig. 3 Experimental protocol of the study. HP-NAP has been delivered systemically (i.p.) (a) or via mucosal route (b). Groups of C57BL/6j mice were treated with saline, with OVA alone, with OVA plus i.p. HP-NAP or with OVA plus mucosal HP-NAP. In both systemic and mucosal protocols, mice were treated with OVA according to a procedure consisting of a first phase of sensitization with OVA i.p. (100 pg/mouse) and a second phase of induction of the allergic response with aerosolized OVA (2% in PBS) for 5 min on day 8, and finally exposed to aerosolized antigen (1% in PBS) for 20 min daily on days 15-18. Control animals, designed as saline, were injected with PBS alone and then exposed to aerosolized PBS. In the systemic protocol (a) mice were treated with i.p. HP-NAP (10 pg/mouse) on day 1, whereas in the mucosal protocol (b) mice received intra-nasal HP-NAP (10 pg/mouse) on days 7 and 8...

Future developments will see the optimization of device design and the investigation of alternative materials for microchip, electrode, and membrane fabrication. It is envisioned that further advances in fabrication and integration procedures will allow the development of implantable/wearable micro-dialysis/microchip systems for personal or on-animal monitoring. Integration of the separation-based systems with powerful detection techniques such as MS will further improve the detection capability of these systems for biological, pharmaceutical, and environmental monitoring. 

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