Analyzing a Lineweaver-Burk plot

The enzyme carbonic anhydrase (Atlas P2) catalyzes the hydration of COj in red blood cells to give bicarbonate (hydrogencarbonate) ion  

The following data were obtained for the reaction at pH = 7.1,273.5 K, and an enzyme concentration of 2.3 nmol dm  

Determine the maximum velocity and the Michaelis constant for the reaction. 

Strategy We construct a Lineweaver-Burk plot by drawing up a table of l/[S]o and l/v. The intercept at 1/ [S]o = 0 is l/f ax and the slope of the line through the points is so Km is found from the slope divided by the intercept. 

The graph is plotted in Fig. 8.4. A lejist-squcffes cuialysis gives ui intercept at 4.00 and a slope of 40.0. It follows that 

---