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Analytical ultracentrifuge, binding studie

Centrifugation can also be used to study macromolecule-ligand interactions, provided that ligand binding alters the sedimentation coefficient of the macromolecule. The analytical ultracentrifuge is well suited for quantitative studies of binding it is a... [Pg.278]

Here we describe studies of the interaction of interleukin-6 (IL-6) with a soluble form of its cell surface receptor (sIL-6R). A procedure utilising a competition approach is presented which allows the determination of the equilibrium constant in solution thus avoiding any potential problems associated with deviation in kinetic characteristics upon surface immobilisation. In addition, binding characteristics of stable monomeric and dimeric forms of IL-6 are presented to demonstrate both the drastic influence of solute multivalency on kinetic and equilibrium properties and the importance of auxiliary techniques such as analytical ultracentrifugation for the interpretation of SPR data. [Pg.418]

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See also in sourсe #XX -- [ Pg.278 ]



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Analytical ultracentrifuge

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