Analytical ultracentrifugation, complexing

Analytical ultracentrifugation (AUC) Molecular weight M, molecular weight distribution, g(M) vs. M, polydispersity, sedimentation coefficient, s, and distribution, g(s) vs. s solution conformation and flexibility. Interaction complex formation phenomena. Molecular charge No columns or membranes required [2]... 

Analyses of the reeonstituted eomplexes by quantitative agarose gel electrophoresis [404,405] and analytical ultracentrifugation [266,406] in the presence of MgCl2 showed that the arrays were able to fold in a way that is almost indistinguishable from complexes reconstituted with major histones (see Fig. 14A-B). Despite this, it was found that histone H2A ubiquitination affects the MgCl2 solubility of the reconstituted complexes (see Fig. 14C) suggesting that this modification may play a role in enhancing the intermolecular associations between chromatin fibers [221]. 

Analytical ultracentrifugation s Equilibrium sedimentation at different car-bohydrate protein ratios yields stoichiometry of complex... 

Karibyants N, Dautzenberg H, Colfen H. Characterization of PSS/ PDADMAC-co-AA polyelectrolyte complexes and their stoichiometry using analytical ultracentrifugation. Macromolecules 1997 30 7803—7809. 

Analytical ultracentrifugation experiments determine shape parameters and the average molecular weight of large molecules in solution, and can thus be used to deduce the stoichiometry of the complexes between hevein domains and... 

---