Analysis of steroid compounds

The various analytical aspects of MS/MS were first investigated with steroids. The identification of compounds, even in complex mixtures, has been performed up to the 

The two aspects of uni- and bimolecular decompositions of ions are used with their respective specificity. These decompositions are detected with both MIKE and linked scan methods. 

In a study of the structure of cholesterol, McLafferty et al. [195] chose to induce the fragmentations by collisions on the molecular ion peak produced by El. They demonstrated the presence of different groups, such as -CHj, -OH. .., on the steroid skeleton studied. 

The following year, Djerassi and co-workers [196] utilized unimolecular decompositions to show the possibility of identifying estrone derivatives (Fig. 42), estrone (a), estradiol (b) and estriol (c). 

The spectra of pure products (reflected spectra) are practically identical to those obtained in the mixture, verifying the specificity of the method. 

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