Analysis of Enzyme Mutants

The site-directed mutagenesis of the nitrilase from P. Jluorescens EBC191 (NitP) focused on specific regions, first of all the C-terminus [25] and the vicinity of the catalytic Cys [26, 27]. 

The C-terminal part of NitP was important for activity, amide formation, enantioselectivity, and stability, as demonstrated by the examination of truncated variants, which generally exhibited lower activities, increased amide formation, altered enantioselectivity, and lowered resistance to freezing and thawing [25]. 

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