Analysis of Anthocyanins in Grape

Where the M+ ions of anthocyanins are evident. Structural identification of compounds is achieved by performing multiple mass 

In general, MSn is also highly effective for differentiation of isobaric compounds. The detection of fragment ions [M-162]+ (Y0+), [M-324]+ (due to consecutive losses of two sugar residues), [M-204]+, [M-308]+ 

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Table 2.4 HPLC gradient program used for analysis of anthocyanins in grape skins extract by C18 (250 x 4mm, 5 pm) column (chromatogram in Figure 2.14) (flow rate 0.5mL/min).

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