Analogs of Adenosylcobalamin

Toraya, T., Miyoshi, S., Mori, M., and Wada, K., 1994, The synthesis of a pyridyl analog of adenosylcobalamin and its coenzymic function in the diol dehydratase reaction, Biochim. Biophys. Acta. 1204 169nl74. 

Numerous analogs of adenosylcobalamin have been tested for their ability to replace or to inhibit the action of the coenzyme in the adenosyl-cobalamin-dependent ribonucleotide reductase reaction the enzyme from L. leichmannii has been used in most of these studies. Kinetic studies have been used in most investigations of analog-enzyme interactions and thus the interpretation of data regarding the affinity of analogs for the reductase is subject to the limitations imposed on kinetic studies of a complex reaction. 

Two recently synthesized analogs of adenosylcobalamin, formycinyl-cobalamin and N6-ethenoadenosylcobalamin may be useful in studies of the mechanism of action of the coenzyme (149). The fluorescence of both nucleosides is completely quenched in the coenzyme form, but after... 

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