Analog-specific Kinases peptide substrates

Ca +ZCaM-dependent kinase activity is also readily measured in low-ionic-strength extracts from rat aortic (Fig. 5B) (Schworer et al., 1993) and swine carotid arterial smooth muscle (Fig. 6) using the peptide substrate autocamtide-2, consistent with a cytosolic distribution. The specific activity of the soluble kinase is approximately one-tenth of that in an analogous fraction of rat or swine forebrain (Schworer et al., 1993), where the average concentration of CaM-kinase II subunits has been estimated to be 1% of total brain protein. On the basis of a native size of 550-650 kDa estimated by gel filtration chromatography, peptide substrate specificity, autophosphorylation properties, and crossreactivity with subunit nonselective anti-CaM-kinase... 

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