Anaerobic continued steady-state

Saccharomyces cerevisiae is anaerobically grown in a continuous culture at 30°C. Glucose is used as substrate and ammonia as nitrogen source. A mixture of glycerol and ethanol is produced. At steady-state condition mass the flow rate is stated. The following reaction is proposed for the related bioprocess 4,6... 

Tests were carried out at 25°C and at initial pH 6.9. Cultures in the liquid medium were incubated in 50 mL Falcon tubes, continuously shaked at 220 rpm. Each culture contained a fresh Pseudomonas sp. 0X1 colony in 10 mL of medium. The airlift with 10 g of pumice was sterilized at 121°C for 30 min and then housed in a sterile room. One-day culture was transferred to the reactor and, after a batch phase, liquid medium with phenol as the only carbon source was continuously fed. The reactor volume V was fixed at 0.13 L. Aerobic conditions were established sparging technical air. Under these conditions microorganism started to grow immobilized on the solid s support. When immobilized biomass approached steady state, cyclic operation of the airlift was started by alternating aerobic/anaerobic conditions. 

Reactions occur in a continuous flow stirred anaerobic reactor without recycle and operating under steady-state conditions. 

With the above constraints, a continuous flow stirred anaerobic reactor as illustrated in Figure 1 will be considered to operate under steady-state conditions dQ/dt = drii°/dt = dni /dt = dn /dt = 0), where Q is the flow rate in liters per day and nf, and represent the number of moles of component Ai per unit time entering the reactor, and leaving the reactor in the gas and aqueous phases, respectively. The system contains m components A (f = 1, 2,. m) interconnected by means of n biologically mediated reactions Bfc (fc = 1, 2,. n). There are more components than reactions so that n is less than m. Sf is the concentration of component A in the reactor in moles per hter. The reactor contains flnite concentrations of all components. 

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