An alternative tissue processing technique

We then asked the question, can we detect free radicals produced during ischemia and reperfusion in myocardial tissues using a more gentle tissue processing technique, namely by chopping of the heart tissues  

The ESR spectra of the control, ischemic, aerobically reperfused and anaerobically reperfused heart samples chopped prior to recording of spectra are shown in Figs. 3A-D. The spectral parameters are given in Table 1. The mass of the frozen tissues in each case is also given in Table 1. 

The amplitude of the ESR signal generated during post-ischemic aerobic reperfusion peaked 10-15 s after initiation of reflow and thereafter gradually declined with time. Reperfusion of ischemic heart with anaerobic perfusate yielded an ESR spectrum identical to that observed at the end of ischemia (compare Figs. 3B and 3D). 

The use of chopped samples indicates marked differences in ESR spectra of the control, ischemic, and post-ischemic reperfused myocardium. Processing 

Perfusion conditions g Peak-to-peak linewidth A//pp (G) Sample size (mg) 

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