AMPPD phenyl

Recently, two major enzyme-catalyzed chemiluminescent reactions have become popular. These use either luminol as a substrate of peroxidase or 3-(2 -spiroadamantane)-4-methoxy-4-(3"-phosphoryloxy)phenyl-1,2-dioxetane (AMPPD) as a substrate of alkaline phosphatase (ALP). 

Substrates for AP are, e.g.,AMPPD (disodium 3-(4-methoxyspi-ro 1 >2-dioxethane-3,2 -ticyclo[3.3.1.1 ] decan -4-yl)phenyl phosphate) or CSPD (disodium 3-(4-methoxyspiro l,2-dioxethane-3,2 -(5"-chloro)tricyclo [3.3.1.1] decan -4-yl)-phenyl phosphate). 

Various 1,2-dioxetane derivatives have been synthesized and reported. Among them, AMPPD (Adamantyl Methoxy Phenyl Phosphoryl Dioxetane) is a well known dioxetane bearing a spiroadamantyl group at the 3-position and a phenol phosphate at the 4-position, which is now used for chemiluminescence enzyme immunoassay (CLEIA) using alkaline phosphatase with highly sensitive detection. 

Using alkaline phosphatase-labeled antibodies to protein antigens and the meta-phenyl phosphate dioxetane shown in Fig. 41 (referred to as AMPPD or Lumigen PPD), one can conduct sandwich-type immunoassays with a solid phase immobilized capture antibody and an enzyme-labeled signal antibody (B19). After the... 

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