Ampholytes variants

To alleviate this waviness enhancing these narrow range ampholytes with small amounts (1 10) of wider range ampholytes (pH 4-5, pH 4-5.5 or pH 3-10) is required (30, 32). In addition to yielding more linear patterns across a gel, enhancing the pH gradient with wider pH range ampholytes does not appear to compromise the separation distances between allelic variant bands. 

Fig. 2 Separation of hemoglobins by CIEF in blood from a patient suffering Hb S/(3+ from thalessemia. Capillary 27 cm (20 cm to detector) X 50 p,m I.D. DB-1 (J W Scientific) ampholytes 2% pH 6-8 10-3 (10 1) Pharmalytes (Pharmaceia Biotech) and 0.375% methylceUulose catholyte 20 mAf sodium hydroxide anolyte 100 mM phosphoric acid in 0.375% methylceUulose focusing 5 min at 30 kV mobilization low pressure (0.5 psi) for 10 min at 30 kV detection UV, 415 nm. Source From Capillary isoelectric focusing of hemoglobin variants in the pediatric clinical laboratory, in Electrophoresis.

---